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. 1999 Aug 2;190(3):385–398. doi: 10.1084/jem.190.3.385

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Western blot analysis of COS-7 cells and DBA/1 fibroblasts engineered to express murine GAL-1. Cell extracts and supernatants of transiently transfected COS-7 cells (lanes C–H) and permanently transfected DBA/1 fibroblasts (lanes I–K) were resolved on a 14% polyacrylamide slab gel, transferred to nitrocellulose, and probed with a 1:500 dilution of an anti–GAL-1 Ab. The immunoreactive profile of rGAL-1 is indicated in lanes A and B (0.75 and 1 μg, respectively). Extracts from COS-7 cells transfected with mGAL-1 (lanes D and E; 20 and 30 μg of transfected DNA, respectively) or control pCDNA3 (lane C) were analyzed for GAL-1 detection in parallel with their supernatants (lane F, pCDNA3; lanes G and H, GAL-1). GAL-1 was highly expressed in cell extracts (lane I) and supernatants (lane J) of transfected syngeneic DBA/1 fibroblasts but not in control transfectants (lane K). Molecular mass standards are shown on the left.