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mCD14–EGFP is expressed on the surfaces of U373 cells. Confocal microscopy was used to collect serial optical sections of a U373–CD14–EGFP cell at 1-μm intervals, beginning at the top of the cell (A–H). A projection of all the sections is shown (P). mCD14–EGFP was localized to the cell surface and Golgi apparatus, consistent with its being expressed and transported to the plasma membrane.
