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. 1999 Aug 16;190(4):509–522. doi: 10.1084/jem.190.4.509

Figure 6.

Figure 6

LPS moves into the cell without mCD14–EGFP. U373–CD14–EGFP cells were incubated in HAP buffer with BODIPY–LPS–sCD14 (40 ng/ml LPS) complexes for 3 min at 37°C, washed, and incubated further for 0, 10, 30, or 60 min at 37°C. Confocal optical sections for mCD14–EGFP and BODIPY–LPS fluorescence are shown for representative cells in the left and center panels, respectively, and the merged images are shown in the right panels. Yellow indicates the regions where the signals overlap. Although there was colocalization of mCD14–EGFP and BODIPY–LPS on the cell surface, internalized BODIPY–LPS did not colocalize with mCD14–EGFP. Bars, 10 μm.