Figure 2.

T cell proliferative response and cytokine production of spleen cells from mice injected with DEF. BALB/c mice adoptively transferred with TCR-HA Tg T cells (3.0 × 10⁷) were injected three times at 24-h intervals with 130 μg/ml DEF or MOPC 173 protein control. 24 h after the last injection, spleen cells (5 × 10⁵) were collected and challenged in vitro with HA110-120 peptide (5 μg/ml), and thymidine incorporation was determined in individual mice (A). Values represent the mean of triplicate wells ± SD (cpm). In the same culture system, the levels of IL-2, IL-4, and IL-10 were determined by ELISA 2 and 3 d after challenge with peptide (B, C, and D, respectively). Values are expressed in pg/ml for individual mice. The percentage of TCR-HA T cells in spleen cells was determined by FACS™ analysis using 6.5.2–FITC conjugate as previously described 20. Animals injected with MOPC 173 protein control showed 30% of TCR-HA T cells in mouse no. 1 and 33% in mouse no. 2, and in those injected with DEF, 44% in mouse no. 1, 43% in mouse no. 2, 41% in mouse no. 3, and 37% in mouse no. 4.