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. 1999 Aug 16;190(4):543–554. doi: 10.1084/jem.190.4.543

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Effect of DEF on CTL and Ab responses. (A) NP147-154–specific CTL activity from mice immunized with live PR8 influenza virus and treated or untreated with DEF on days 7, 8, and 9 after immunization. Spleen cells collected on day 10 after immunization were stimulated in vitro for 5 d with NP147-154 peptide, and CTL activity was measured against P18 target cells coated with NP147-154 synthetic peptide. The percentage of cytotoxicity was determined in four mice and corresponds to the mean of triplicate samples (cpm) ± SD. (B) IgG2a and IgG1 anti-PR8 Ab titers in BALB/c mice previously immunized with live influenza PR8 virus and then injected three times with 130 μg of DEF or PBS on days 7, 8, and 9 after immunization. Values represent the mean cpm measured in triplicate blood samples ± SD collected from individual mice on days 0, 7, and 14 after immunization.

Effect of DEF on CTL and Ab responses. (A) NP147-154–specific CTL activity from mice immunized with live PR8 influenza virus and treated or untreated with DEF on days 7, 8, and 9 after immunization. Spleen cells collected on day 10 after immunization were stimulated in vitro for 5 d with NP147-154 peptide, and CTL activity was measured against P18 target cells coated with NP147-154 synthetic peptide. The percentage of cytotoxicity was determined in four mice and corresponds to the mean of triplicate samples (cpm) ± SD. (B) IgG2a and IgG1 anti-PR8 Ab titers in BALB/c mice previously immunized with live influenza PR8 virus and then injected three times with 130 μg of DEF or PBS on days 7, 8, and 9 after immunization. Values represent the mean cpm measured in triplicate blood samples ± SD collected from individual mice on days 0, 7, and 14 after immunization.