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. 1999 Aug 16;190(4):585–590. doi: 10.1084/jem.190.4.585

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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CRP binds to COS–7 cells transfected with the FcγRIIA cDNA, and to K-562 cells with similar kinetics. Cells were incubated with increasing doses of CRP and binding was detected with 2C10 and PE-GAM, as described in Materials and Methods. Results are expressed as the increase in gMCF compared with the secondary antibody controls. (A) COS-7 cells transfected with the FcγRIIA plasmid or pcDSRα296, or mock-transfected. Specific binding was determined by subtracting gMCF of mock-transfected cells from gMCF of transfected cells. (B) K-562 cells.