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. 1999 Aug 16;190(4):585–590. doi: 10.1084/jem.190.4.585

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Analysis of CRP binding to transfected COS-7 cells by two-color flow cytometry. COS-7 cells were transfected with the FcγRIIA plasmid, pcDSRα296. Mock-transfected cells received no DNA. Cells were incubated with 200 μg/ml of CRP and binding was detected with 2C10 and PE-GAM as described in Materials and Methods. Levels of FcγRII were determined by binding of FITC-AT10. A, B, C, and D, transfected; E, F, G, and H, mock-transfected. A and E, staining with 2C10 and PE-GAM; B and F, staining for CD-32 only; C and G, staining for CRP only; D and H, staining for CRP and CD32.