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. 1999 Aug 16;190(4):585–590. doi: 10.1084/jem.190.4.585

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Treatment of U-937 cells with Bt₂cAMP increases CRP binding. U-937 cells were grown for 60 h in medium containing 1 mM Bt₂cAMP before analysis of CRP binding. CRP binding was detected with 2C10 and PE-GAM as described in Materials and Methods. Results are expressed as the increase in gMCF compared with the secondary antibody controls. (Inset) FcγRI and FcγRII levels were measured using mAb 32.2 and PE-F(ab′)₂ GAM IgG and PE-C1KM5, respectively.