Figure 3.

EC induction of CD86 on CD4⁺ T cells. (A) Double stain FACS^® analysis of resting CD4⁺ T cells, or CD4⁺ T cells that were cultured with IFN-γ–activated ECs for 72 h using FITC-conjugated CD3 and PE-conjugated CD86 antibodies (PE-conjugated mouse isotype antibody served as control). (B) The expression of CD86 by semiquantitative RT-PCR on RNA harvested from CD4⁺ T cells alone (lane 1), CD4⁺ T cells cultured with cell membrane preparations from unactivated ECs (lane 2), or CD4⁺ T cells cultured with cell membrane preparations from IFN-γ–treated ECs (lane 3). (C) CD86 and E-selectin expression by RT-PCR on RNA harvested from HUVECs (lane 1), HUVECs cultured with cell membrane preparations from resting CD4⁺ T cells (lane 2), or HUVECs cultured with cell membrane preparations from PHA-activated CD4⁺ T cells (lane 3). IFN-γ–treated monocytes were used as a positive control (lane 4). Membranes were prepared as described in Materials and Methods from 5 x10⁶ cells. Data is representative of three similar experiments.