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. 1999 Aug 16;190(4):555–566. doi: 10.1084/jem.190.4.555

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Effect of CD86 trans-costimulation on alloantigen-induced CD4⁺ T cell activation. (A) CD4⁺ T cells (5 × 10⁵) were cocultured with IFN-γ–treated endothelial cells in the presence of increasing numbers of either CD86-transfected or mock-transfected CHO cells. CHO cells were fixed in 0.4% paraformaldehyde before coculture to inhibit spontaneous proliferation. (B) Anti-CD86 (10 μg/ml, IT2.2) or CTLA4 Ig (10 μg/ml) were added to cocultures as indicated. Proliferation was assessed by [³H]thymidine incorporation for the final 18 h of a 6-d culture. Shown is one experiment representative of four in triplicate cultures ± 1 SD. CD4⁺ T cells cocultured with CHO cells alone did not proliferate.

Effect of CD86 trans-costimulation on alloantigen-induced CD4⁺ T cell activation. (A) CD4⁺ T cells (5 × 10⁵) were cocultured with IFN-γ–treated endothelial cells in the presence of increasing numbers of either CD86-transfected or mock-transfected CHO cells. CHO cells were fixed in 0.4% paraformaldehyde before coculture to inhibit spontaneous proliferation. (B) Anti-CD86 (10 μg/ml, IT2.2) or CTLA4 Ig (10 μg/ml) were added to cocultures as indicated. Proliferation was assessed by [³H]thymidine incorporation for the final 18 h of a 6-d culture. Shown is one experiment representative of four in triplicate cultures ± 1 SD. CD4⁺ T cells cocultured with CHO cells alone did not proliferate.