Figure 8.

Functional effects of resting and EC-modified monocytes. (A) Monocytes were cultured on a monolayer of IFN-γ–treated ECs or a plastic culture dish as a control for 72 h and were reisolated by positive selection using CD14-coated magnetic beads. Monocytes were then irradiated (1,750 rads) and added at fixed ratios to either allogeneic or autologous CD4⁺ T cells (10⁵) for 6 d. (B) Resting or EC-modified monocytes (as above) were cocultured with allogeneic CD4⁺ T cells in a mixed leukocyte reaction (stimulator/responder ratio 1:10). Anti–HLA-DR (10 μg/ml) or CTLA4 Ig (10 μg/ml) were added to cultures. Proliferation was assessed by [³H]thymidine incorporation after 6 d of coculture. Results shown are representative of three experiments in triplicate cultures ± 1 SD.