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. 1999 Aug 16;190(4):523–534. doi: 10.1084/jem.190.4.523

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Quantification of LPS with lysosomal and Golgi apparatus markers in neutrophils. Cells were labeled in the presence of either LysoTracker™ Red DND-99 or TRITC–CTB for 30 min at 37°C and processed for microscopy. 15–40 fields of live cells per experiment were observed, with an average of 5–20 cells per field. All green (LPS-containing) vesicles in the field were visually assigned as either not colocalized or colocalized with the red Golgi apparatus or lysosomal marker. Each experiment included data on 101–245 vesicles. Data is presented as the mean ± range for two experiments.