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. 1999 Aug 16;190(4):523–534. doi: 10.1084/jem.190.4.523

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Localization of LPS in HeLa cells is disrupted by BFA. HeLa cells were incubated with BODIPY–LPS and TRITC–CTB for 60 min at 37°C in DME with HSA, washed, and warmed in the absence or presence of BFA (1 μg/ml) for 60 min at 37°C before examination by confocal microscopy. LPS (green) colocalizes with CTB (red) and appears in a perinuclear area before BFA treatment (yellow on merge panels). Upon BFA exposure, however, BODIPY–LPS and TRITC–CTB redistribute to scattered/fragmented cytoplasmic structures.

Localization of LPS in HeLa cells is disrupted by BFA. HeLa cells were incubated with BODIPY–LPS and TRITC–CTB for 60 min at 37°C in DME with HSA, washed, and warmed in the absence or presence of BFA (1 μg/ml) for 60 min at 37°C before examination by confocal microscopy. LPS (green) colocalizes with CTB (red) and appears in a perinuclear area before BFA treatment (yellow on merge panels). Upon BFA exposure, however, BODIPY–LPS and TRITC–CTB redistribute to scattered/fragmented cytoplasmic structures.