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. 1999 Sep 6;190(5):651–668. doi: 10.1084/jem.190.5.651

Table 2.

Frequency of Peptide-specific CTLp Directed to Melan-A/Mart-127–35 Peptide in Peripheral Blood of Melanoma Patients

Patient Frequency of CTLp to Melan-A/Mart-127–35 peptide in
PBLs CD45RO+ T cell subset CD45RA+ T cell subset
1 1/1,404 ND ND
2 1/1,708 1/1,118 1/56,787
6 1/1,399 1/879 <1/200,000
9 1/2,225 ND ND
3 1/43,717 ND ND
4 1/42,600 ND ND
5 1/117,555 ND ND
7 <1/200,000 <1/200,000 1/42,919
8 1/96,691 <1/200,000 1/22,430

CTLp frequency was evaluated in peripheral blood by LDA as described in Materials and Methods by using peptide-loaded T2 cells as APCs. Results are expressed as peptide-specific CTL precursors/PBLs. In patients 6 and 9, frequency of Melan-A/Mart-1–specific T cells in PBLs was 1/1,456 and 1/1,843, respectively, by staining with HLA-A*0201–Melan-A/Mart-126–35 tetrameric complexes.