Table 4.

Frequency of Melan-A/Mart-1_27–35–specific CTL Effectors on Day 42 of Bulk Culture after T Cell Activation with Different APCs

APCs	Peptide used to load APCs in bulk culture	Frequency, after bulk culture, of peptide-specific CTL effectors directed to
		Melan-A/Mart-127–35 peptide	Influenza matrix58–66 peptide
T2 cells	Melan-A/Mart-127–35	1/69	ND
CD34+-derived autologous DCs	Melan-A/Mart-127–35	1/63	ND
Monocyte-derived autologous DCs	Melan-A/Mart-127–35	1/142	ND
Fresh autologous monocytes	Melan-A/Mart-127–35	<1/200,000	ND
Fresh autologous monocytes	Flu matrix58–66		1/1,190

Frequency of Melan-A/Mart-1_27–35- and Flu matrix_58–66–specific effectors was determined respectively on days 42 and 21 of bulk T cell culture. Responder lymphocytes were from one of the patients (patient 7) in the low CTLp frequency subset. Phenotype of all APCs was checked by flow cytometry. CD34⁺ and monocyte-derived DCs expressed CD1a, CD40, CD80, CD86, CD54, HLA-DR, and HLA-DQ antigens. T2 cells expressed CD40, CD80, CD86, and CD54. CD14 was expressed only by monocytes, which were also positive for HLA-DR and CD54 but lacked CD40, CD80, CD86, and HLA-DQ antigens.