Table 8.
Immunohistochemical Analysis of Melanoma Lesions
| Patient | Date | CTLp | Lesion number | Tumor sample | Evidence of tumor regression | HLA class I on fresh tumor | HLA-A2 on fresh tumor | CD3+ lymphocyte infiltrate | CD8+ lymphocyte infiltrate | Melan-A/Mart-1 expression | |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Positive cells | Staining intensity | ||||||||||
| d/m/y | % | % | |||||||||
| 1 | 04/02/91 | 1 | PR | Necr. (5%) | Absent | 20 | +++ (50%) | ||||
| + (50%) | |||||||||||
| 04/02/91 | 2 | PR | No | Nonbrisk | 30 | 0 | |||||
| 27/03/91 | 3 | s.c. met. | No | 95/371 | 91/225 | Nonbrisk | 30 | 90 | |||
| 29/03/91 | 4 | LN met. | Necr. (1%) | Absent | <1 | + | |||||
| 04/04/91 | High | ||||||||||
| 24/10/91 | 5 | s.c. met. | No | Brisk | 100 | 50 | + (20%) | ||||
| +/− (80%) | |||||||||||
| 24/10/91 | 6 | s.c. met. | No | Brisk | 95 | 0 | |||||
| 24/10/91 | 7 | s.c. met. | No | Brisk | 95 | 40 | + (20%) | ||||
| +/− (80%) | |||||||||||
| 2 | 29/05/90 | 8 | PR | NA | NA | NA | |||||
| 04/07/91 | High | ||||||||||
| 10/07/91 | 9 | LN met. | Necr. (50%) | 100/312 | 92/170 | Nonbrisk | 100 | 15 | +++ (20%) | ||
| + (80%) | |||||||||||
| 6 | 11/80 | 10 | PR | NA | NA | NA | |||||
| 23/01/92 | High | ||||||||||
| 27/01/92 | 11 | S.T. met. | Scler. (10%) | 100/69 | 89/56 | Absent | 0 | ||||
| 9 | 28/10/87 | 12 | PR | Scler. (10%) | 0 | 0 | Absent | 70 | +++ (60%) | ||
| ++ (40%) | |||||||||||
| 28/10/87 | 13 | s.c. met. | No | 0 | 0 | Absent | 50 | +++ (30%) | |||
| ++ (70%) | |||||||||||
| 28/10/87 | 14 | LN met. | No | 0 | 0 | Absent | 80 | +++ (70%) | |||
| 21/04/88 | High | ++ (30%) | |||||||||
| 3 | 23/04/91 | 15 | PR | No | Absent | 5 | + (100%) | ||||
| 14/10/91 | Low | ||||||||||
| 14/10/91 | 16 | LN met. | Necr. (30%) | 94/80 | 95/32 | Absent | 20 | +++ (70%) | |||
| ++ (30%) | |||||||||||
| 14/10/91 | 17 | LN met. | Necr. (1%) | Absent | 5 | +++ (100%) | |||||
| 4 | 16/12/85 | 18 | PR | No | 100/169 | 82/83 | Nonbrisk | 50 | <1 | + | |
| 16/12/85 | 19 | LN met. | H.Necr. (1%) | Nonbrisk | 20 | 0 | |||||
| 19/01/87 | 20 | S.T. met. | H.Necr. (1%) | Absent | 0 | ||||||
| 19/01/87 | 21 | S.T. met. | No | Absent | 0 | ||||||
| 29/06/87 | Low | ||||||||||
| 5 | 09/93 | 22 | PR | No | ND | 80 | Absent | 0 | |||
| 08/11/95 | 23 | s.c. met. | Scler. (30%) | 100/98 | 95/118 | Absent | 20 | +++ (90%) | |||
| ++ (10%) | |||||||||||
| 10/11/95 | Low | ||||||||||
| 17/10/96 | 24 | LN met. | H.Necr. (5%) | Absent | 60 | ++ (40%) | |||||
| Scler. (5%) | + (60%) | ||||||||||
| 7 | 24/09/91 | 25 | PR | No | Absent | 20 | +++ | ||||
| 27/01/93 | 26 | LN met. | H.Necr. (25%) | Brisk | 100 | <1 | + | ||||
| Scler. (15%) | |||||||||||
| 04/03/96 | Low | ||||||||||
| 8 | 31/08/91 | 27 | PR | No | Nonbrisk | 100 | 70 | ++ (30%) | |||
| + (70%) | |||||||||||
| 11/12/91 | 28 | LN met. | Scler. (20%) | Brisk | 50 | 30 | ++ (95%) | ||||
| 19/02/96 | Low | +++ (5%) | |||||||||