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. 1999 Sep 6;190(5):681–690. doi: 10.1084/jem.190.5.681

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Expression of chemokine receptors in me^v/me^v mice compared with +/+ mice. Freshly isolated bone marrow cells (BM), spleen mononuclear cells (Spleen), and CD4⁺ T cells (purity >95%) from +/+ and me^v/me^v mice were analyzed for expression of chemokine receptor mRNA by RNase protection assay. L32 and GAPDH were included as internal controls to normalize expression of chemokine receptors in different mice and cell types. Expression of CXC chemokine receptors is shown in A and that of CC chemokine receptors is shown in B. Each band's radiointensity was measured by phosphor-image analyzer (Molecular Image Analyzer; Bio-Rad) to quantitate expression of chemokine receptors. Normalized expression levels of chemokine receptors for L32 internal control (expression of chemokine receptor/expression of L32) from three independent experiments were averaged, and are shown in C.

Expression of chemokine receptors in me^v/me^v mice compared with +/+ mice. Freshly isolated bone marrow cells (BM), spleen mononuclear cells (Spleen), and CD4⁺ T cells (purity >95%) from +/+ and me^v/me^v mice were analyzed for expression of chemokine receptor mRNA by RNase protection assay. L32 and GAPDH were included as internal controls to normalize expression of chemokine receptors in different mice and cell types. Expression of CXC chemokine receptors is shown in A and that of CC chemokine receptors is shown in B. Each band's radiointensity was measured by phosphor-image analyzer (Molecular Image Analyzer; Bio-Rad) to quantitate expression of chemokine receptors. Normalized expression levels of chemokine receptors for L32 internal control (expression of chemokine receptor/expression of L32) from three independent experiments were averaged, and are shown in C.