Figure 2.

Inhibition of antibody binding to P-selectin. CHO-P cells were incubated in increasing concentrations of glycocalicin or soybean trypsin inhibitor before the addition of anti–P-selectin polyclonal antibody. The binding of the antibody was evaluated by flow cytometry after incubation of the cells with an FITC-conjugated goat anti–rabbit secondary antibody. 100% binding is the binding in the absence of added protein. Glycocalicin inhibited antibody binding, with an IC₅₀ of ∼25 μg/ml. Soybean trypsin inhibitor had no effect. Values reported are averaged from three experiments.