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. 1999 Sep 20;190(6):861–874. doi: 10.1084/jem.190.6.861

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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β3 integrin and αvβ5 integrin localize to attached and free macrophage plasma membrane. β3 integrins but not αvβ5 integrin redistribute to attachment sites in macrophages seeded on fibrinogen substrate. Immunodetection of surface integrins was performed on unpermeabilized cells with β3-specific or αvβ5 receptor–specific antibodies. Top and bottom panels shown are en face views of the apical and the attached cell surface, respectively. (a) β3 and αvβ5 integrin distribution in J774 macrophages attached to laminin-coated coverslips for 30 min. β3 and αvβ5 are present on both surfaces, as demonstrated by the immunofluorescence signals in horizontal optical sections at the levels of attached (a1 and a3) and free (a2 and a4) cell surfaces. (b) Same staining as in a, of J774 macrophages seeded on fibrinogen for 30 min, reveals specific relocalization of β3 integrins to attachment sites. Compare a1 with b1, a2 with b2. Scale bars, 10 μm.