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. 1999 Oct 4;190(7):1013–1024. doi: 10.1084/jem.190.7.1013

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Addition of IL-2 restores the proliferative capacity of aged Tg⁺ CD4 cells. (A) 3-d [³H]TdR incorporation assay of young (circles) and aged (squares) Tg⁺ CD4 cells stimulated with DCEK-ICAM APCs and 5 μM PCCF with (open symbols) and without (filled symbols) exogenous IL-2. [³H]TdR was added for the last 16 h of culture. Data is representative of three experiments. (B) Tg⁺ CD4 cells from young (circles) and aged (squares) mice were stimulated with DCEK-ICAM APCs at 2:1 T cell/APC and 5 μM PCCF with (open symbols) or without (filled symbols) exogenous IL-2 in a 24-well plate. At each time point, cell counts were performed. Data is representative of three experiments.