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. 1999 Oct 4;190(7):1013–1024. doi: 10.1084/jem.190.7.1013

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Cytokine production by young and aged Th1 and Th2 effectors. Effectors were generated from young and aged Tg⁺ CD4 cells with DCEK-ICAM APCs (2:1 T cell/APC), PCCF, and Th1 or Th2 polarizing cytokines and anticytokine antibodies (see Materials and Methods). All supernatants (24 h) were collected from 3 × 10⁵ day 4 effectors restimulated with DCEK-ICAM and PCCF and assayed for IL-2, IL-4, IL-5, and IFN-γ. Data is representative of three experiments.