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. 1999 Oct 4;190(7):995–1004. doi: 10.1084/jem.190.7.995

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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IFN-γ and TNF-α production by LP CD4⁺ T cells from colons. RAG-2^−/− or C.B-17 SCID recipients were reconstituted with cell subsets as indicated. 8–12 wk after cell reconstitution, LP cells were isolated and stimulated for 12 h with anti-CD3∈ antibody. Levels of cytokine expression were determined by cytofluorography. (A) Absolute number of CD4⁺ cytokine-positive cells per colon. Numbers were determined by multiplying the frequency of cytokine-secreting CD4⁺ T cells by the total number of CD4⁺ cells. Data represent the mean ± SEM of two to five animals per group. (B) Frequency of cytokine-secreting CD4⁺ T cells. Data are gated on CD4⁺ T cells and are representative examples for each group. Horizontal lines represent staining with isotype control mAb.

IFN-γ and TNF-α production by LP CD4⁺ T cells from colons. RAG-2^−/− or C.B-17 SCID recipients were reconstituted with cell subsets as indicated. 8–12 wk after cell reconstitution, LP cells were isolated and stimulated for 12 h with anti-CD3∈ antibody. Levels of cytokine expression were determined by cytofluorography. (A) Absolute number of CD4⁺ cytokine-positive cells per colon. Numbers were determined by multiplying the frequency of cytokine-secreting CD4⁺ T cells by the total number of CD4⁺ cells. Data represent the mean ± SEM of two to five animals per group. (B) Frequency of cytokine-secreting CD4⁺ T cells. Data are gated on CD4⁺ T cells and are representative examples for each group. Horizontal lines represent staining with isotype control mAb.