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. 1999 Oct 4;190(7):935–942. doi: 10.1084/jem.190.7.935

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Tethering and rolling of Jurkat cells on various GlyCAM-1/IgG chimeras over a range of site densities. Jurkat cells (2 × 10⁶ cells/ml) were perfused into the chamber at 1 dyn/cm², and the frequency of stable tethers (A) and the velocity of cells (B) was determined after 2 min of flow. Data points represent the mean ± SD (A) and the mean ± SEM (B) in three independent experiments, each performed at least in duplicate. Statistical analysis using an unpaired two-tailed Student's t test showed that the enhanced tethering frequencies and slower velocities of Jurkat cells on sulfated GlyCAM-1/IgG as compared with nonsulfated GlyCAM-1/IgG were statistically significant (for KSGal6ST, P < 0.0001 [A and B] at all site densities; for HEC-GlcNAc6ST at 33.3 and 100%, P < 0.008 [A] and P < 0.005 [B]).

Tethering and rolling of Jurkat cells on various GlyCAM-1/IgG chimeras over a range of site densities. Jurkat cells (2 × 10⁶ cells/ml) were perfused into the chamber at 1 dyn/cm², and the frequency of stable tethers (A) and the velocity of cells (B) was determined after 2 min of flow. Data points represent the mean ± SD (A) and the mean ± SEM (B) in three independent experiments, each performed at least in duplicate. Statistical analysis using an unpaired two-tailed Student's t test showed that the enhanced tethering frequencies and slower velocities of Jurkat cells on sulfated GlyCAM-1/IgG as compared with nonsulfated GlyCAM-1/IgG were statistically significant (for KSGal6ST, P < 0.0001 [A and B] at all site densities; for HEC-GlcNAc6ST at 33.3 and 100%, P < 0.008 [A] and P < 0.005 [B]).