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. 1999 Oct 4;190(7):935–942. doi: 10.1084/jem.190.7.935

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Resistance of rolling Jurkat cells to shear-induced detachment on various GlyCAM-1/IgG chimeras. Jurkat cells (2 × 10⁶ cells/ml) were allowed to tether onto different GlyCAM-1/IgG chimeras at a wall shear stress of 1 dyn/cm² for 2 min. Wall shear stress was then increased at intervals of 5 s to a maximum of 35 dyn/cm², and the number of rolling cells remaining bound at each shear stress was determined and expressed as the percentage of the maximum number of adherent cells. The data points represent the mean ± SD of three to four independent experiments, each performed in duplicate in two different fields of view. A statistical comparison of the detachment curves using a two-factor analysis of variance showed that the increased binding to sulfated GlyCAM-1/IgG as compared with nonsulfated GlyCAM-1 was statistically significant (for KSGal6ST, P < 0.0001; for huGlcNAc6ST, P < 0.02; and for HEC-GlcNAc6ST, P < 0.0003).