Table 3.
Transfection | Rolling velocity (μm/s) | Shear stress for 50% detachment | Tethering frequency at 0.4 dyn/cm2 | ||
---|---|---|---|---|---|
1 | 8 | 20 | |||
dyn/cm2 | dyn/cm2 | % | |||
FT | 3.7 ± 0.9 | 7.6 ± 1.7 | 13.5 ± 2.7 | 15.0 ± 3.4 | 61.4 ± 4.3 |
FT plus KSGal6ST | 4.0 ± 0.8 | 7.3 ± 1.5 | 11.5 ± 2.0 | 15.2 ± 1.2 | 61.8 ± 3.8 |
FT plus huGlcNAc6ST | 4.3 ± 0.9 | 8.8 ± 1.5 | 13.4 ± 2.6 | 11.4 ± 0.9 | 59.9 ± 6.4 |
FT plus HEC-GlcNAc6ST | 4.4 ± 0.9 | 7.6 ± 1.8 | 11.9 ± 1.8 | 17.1 ± 2.6 | 63.7 ± 8.3 |
Statistical analysis (unpaired two-tailed Student's t test) comparing sulfated (FT plus ST) versus nonsulfated (FT) GlyCAM-1/IgG substrates showed no significant differences.