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. 1999 Oct 4;190(7):935–942. doi: 10.1084/jem.190.7.935

Table 3.

Rolling of E-Selectin/300.19 Cells on Various GlyCAM-1/IgG Chimeras

Transfection Rolling velocity (μm/s) Shear stress for 50% detachment Tethering frequency at 0.4 dyn/cm2
1 8 20
dyn/cm2 dyn/cm2 %
FT 3.7 ± 0.9 7.6 ± 1.7 13.5 ± 2.7 15.0 ± 3.4 61.4 ± 4.3
FT plus KSGal6ST 4.0 ± 0.8 7.3 ± 1.5 11.5 ± 2.0 15.2 ± 1.2 61.8 ± 3.8
FT plus huGlcNAc6ST 4.3 ± 0.9 8.8 ± 1.5 13.4 ± 2.6 11.4 ± 0.9 59.9 ± 6.4
FT plus HEC-GlcNAc6ST 4.4 ± 0.9 7.6 ± 1.8 11.9 ± 1.8 17.1 ± 2.6 63.7 ± 8.3

Statistical analysis (unpaired two-tailed Student's t test) comparing sulfated (FT plus ST) versus nonsulfated (FT) GlyCAM-1/IgG substrates showed no significant differences.