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. 1999 Oct 18;190(8):1093–1102. doi: 10.1084/jem.190.8.1093

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Pre-TCR expression on the surface of the SCB29 cell line and comparison of the labeling intensity between streptavidin–PBXL-3 and streptavidin–FITC. SCB29 cells were initially incubated with biotinylated 2F5 (pTα) and H57 (pan-TCR-α/β) antibodies (thick line) or isotype-matched controls (thin line), and surface expression was revealed by streptavidin conjugated to the secondary reagents. Similar results were obtained in two other experiments.

Pre-TCR expression on the surface of the SCB29 cell line and comparison of the labeling intensity between streptavidin–PBXL-3 and streptavidin–FITC. SCB29 cells were initially incubated with biotinylated 2F5 (pTα) and H57 (pan-TCR-α/β) antibodies (thick line) or isotype-matched controls (thin line), and surface expression was revealed by streptavidin conjugated to the secondary reagents. Similar results were obtained in two other experiments.