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. 1999 Oct 18;190(8):1093–1102. doi: 10.1084/jem.190.8.1093

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Flow cytometric analysis of thymocytes from TCR-transgenic SLP-76^−/− and SLP-76^+/− littermates. Surface expression on thymocytes of (A) CD3 (anti-CD3∈–PE) versus TCR-β (anti–TCR-β–FITC), (B) CD4 (anti-CD4–FITC) versus CD8 (anti-CD8–PE), and (C) CD44 and CD25 on DN thymocytes from SLP-76^−/− and SLP-76^+/− littermates. Cells were triple stained with anti-CD44–FITC, anti-CD25–PE, and a cocktail of biotin-conjugated mAbs to CD3, CD4, CD8, B220, Mac-1, and Gr-1, followed by streptavidin–Cy-Chrome. Analysis was performed on gated Cy-Chrome–negative cells. The percentage of cells found in each quadrant is indicated. In all FACS™ analyses, results from SLP-76^+/− and WT mice were similar. Therefore, only data of SLP-76^+/− mice is shown. Results are representative of four mice examined in each group.