Figure 4.

Characterization of GPR-9-6 expression on memory and naive CD4 lymphocytes: Mononuclear cells were stained in four-color experiments using anti-CD4–TC and anti-CD45RO–allophycocyanin to gate on memory and naive CD4 lymphocytes. The cells were also stained with anti–GPR-9-6 mAb 3C3 followed by F(ab)₂ anti–mouse IgG–PE or –FITC to study GPR-9-6 expression on subsets defined with dye-linked mAbs: anti-αE–FITC (HML1), anti-β7–PE (Fib504), anti-CD49d–PE (HP2/1), anti-CLA–FITC (HECA 452), and anti-CD62L–PE (Dreg 56). Staining with an isotype control IgG_2b mAb followed by F(ab)₂ anti–mouse IgG–PE/FITC was performed and gave FL1/FL2 values <10 U. The percentage of cells falling within each quadrant is shown in the upper right. To directly compare the levels of GPR-9-6 on naive and memory subsets defined by expression of GPR-9-6, we show the ratio of the mean fluorescence intensity for memory/naive subset for the relevant quadrants (Q) (n = 3).