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. 1999 Nov 15;190(10):1505–1516. doi: 10.1084/jem.190.10.1505

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Cytofluorimetric analysis of the NKp30 molecules expressed in COS-7 cell transfectants; amino acid sequence and hydrophobicity plot of NKp30. (A) COS-7 cells transfected with clone 5C cDNA construct were stained with anti-NKp30 (A76, AZ20, and Z25) or with anti-NKp46 (BAB281) mAbs followed by PE-conjugated goat anti–mouse IgG1, and analyzed by flow cytometry. White profiles represent cells incubated with the second reagent alone (i.e., negative controls). (B) The putative signal peptide is indicated in lowercase letters, and the transmembrane region is underlined. Cysteines involved in the Ig-like fold are circled, and putative N-glycosylation sites are boxed. A Kyte-Doolittle hydrophobicity plot is shown at the bottom. DNA and protein sequence analyses were performed using GeneWorks, MacVector suites, NetOGlyc version 2.0 (available at http://www.cbs.dtu.dk/services/NetOGlyc), and PSORT Prediction Servers (available at http://psort.nibb.ac.jp:8800/). The sequence data are available from EMBL/GenBank/DDBJ under accession no. AJ223153.

Cytofluorimetric analysis of the NKp30 molecules expressed in COS-7 cell transfectants; amino acid sequence and hydrophobicity plot of NKp30. (A) COS-7 cells transfected with clone 5C cDNA construct were stained with anti-NKp30 (A76, AZ20, and Z25) or with anti-NKp46 (BAB281) mAbs followed by PE-conjugated goat anti–mouse IgG1, and analyzed by flow cytometry. White profiles represent cells incubated with the second reagent alone (i.e., negative controls). (B) The putative signal peptide is indicated in lowercase letters, and the transmembrane region is underlined. Cysteines involved in the Ig-like fold are circled, and putative N-glycosylation sites are boxed. A Kyte-Doolittle hydrophobicity plot is shown at the bottom. DNA and protein sequence analyses were performed using GeneWorks, MacVector suites, NetOGlyc version 2.0 (available at http://www.cbs.dtu.dk/services/NetOGlyc), and PSORT Prediction Servers (available at http://psort.nibb.ac.jp:8800/). The sequence data are available from EMBL/GenBank/DDBJ under accession no. AJ223153.