Table 1.
Primer Sequences Used in V Region–specific PCR Amplifications
| V region primers | |
|---|---|
| VH-SG1 | GTGCAGCTKMAGSAGTCRGG |
| VH-SG2 | CARCTGCARCARYCTGG |
| VH-SG3 | GTGAAGCTKSWSGARTCTGG |
| VH-SG4 | GTYCARCTKCARCAGTCTGG |
| VH13-3-5′ | GCTGAACTGGCAAAACCT |
| VH13-3-3′ | GTAATAGACTGCAGAGTC |
| Vκ1-FW1 | AGTCTTGGAGATCAAGCC |
| Vκ24-CDR1 | TCCTGCAGGTCTAGTAAG |
| Vκ24-FW3 | GCCTCAGGAGTCCCAGAC |
| Vκ1,2 | ATGTTGTGATGACCCA |
| Vκ4,5 | AAATTGTTCTCACCCA |
| Vκ8,14,15,19,21 | ACATTGTGMTGACHCA |
| Vκ9,12,13,31,33,34 | ACATCCAGATGACHCA |
| Vκ20 | AAACAACTGTGACCCA |
| Vkκ23 | ATATTGTGCTAACTCA |
| Vκ24,28 | RTATTGTGATGACYCA |
| Vλ | TTCCCAGGCTGTTGTGA |
| C region primers | |
| JH3 | GCAGAGAATCTTGGTCCT |
| IgG3 | GTCACTGCAGCCAGGGACCA |
| IgA | GATGGTGGGATTTCTCGCAGACTC |
| IgGX | CAGGGGCCAGTGGATAGAC |
| Jκ2 | CCTTAACACTTGATCTGA |
| Jκ4 | CTATTGATGCACAGGTTG |
| Cκ | ACACTCATTCCTGTTGAA |
| Cλ | GAGCTCTTCAGAGGAAGG |