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. 1999 Jun 8;96(12):6581–6586. doi: 10.1073/pnas.96.12.6581

Figure 1.

Figure 1

(A) Dissociation kinetics of F-PCC-(89–104)/I-Ek in PBS at 25°C in the presence of various concentrations of dynA-(1–13). ●, No dynA; □, 1.5 μM dynA (100-fold molar excess); ♦, 15 μM dynA (1,000-fold molar excess); ×, 75 μM dynA (5,000-fold molar excess); ○, 150 μM dynA (10,000-fold molar excess); ■, 300 μM dynA (20,000-fold molar excess). Data were acquired until the fluorescence signal of each dissociation reaction [F(t)] was smaller than 5% of the fluorescence at time zero (F0). For clarity, data points are shown only up to 40 hr. (B) Percent observed fast-dissociating complex versus molar ratio of dynA-(1–13) to F-PCC-(89–104)/I-Ek. Values were taken from the corresponding dissociation curves in A. Error bars represent the standard deviation of two independent dissociation experiments.