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FRET of 10 nM F-PCC-(89–104)/I-Ek in the presence of various concentrations of TR-dynA-(1–13)/dynA-(1–13) (1:50 molar ratio) in PBS at room temperature. To account for the emission of TR-dynA-(1–13) at 612 nm by exciting at 492 nm [excitation maximum of fluorescein (F)], measured counts were divided by the counts of TR-dynA-(1–13)/dynA-(1–13) (1:50) in the absence of F-PCC-(89–104)/I-Ek under identical conditions.
