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. 1999 Nov 15;190(10):1383–1392. doi: 10.1084/jem.190.10.1383

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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CD2–CD48 and LFA-1–ICAM-1 enhance Ca²⁺ fluxes at low antigen concentration. Thioglycollate-elicited macrophages derived from control or ICAM-1–deficient mice were pulsed with various doses of peptide p33, mixed with INDO-1–pulsed, purified CD8⁺ T cells derived from TCR-transgenic control or CD2-deficient mice, and centrifuged together. Elevation of [Ca²⁺]_i was assessed by measuring the FL5/FL4 ratio. (A) FL5/FL4 ratio is shown after stimulation with 10⁻¹¹ M p33-pulsed macrophages. (B) Mean FL5/FL4 ratios are shown as a function of the peptide concentration for the various combinations. Baseline FL5/FL4 values were subtracted for the calculation. One representative experiment of two is shown. ▪, CD2⁺ ICAM⁺; •, CD2⁻ICAM⁺; □, CD2⁺ICAM⁻; ○, CD2⁻ICAM⁻.

CD2–CD48 and LFA-1–ICAM-1 enhance Ca²⁺ fluxes at low antigen concentration. Thioglycollate-elicited macrophages derived from control or ICAM-1–deficient mice were pulsed with various doses of peptide p33, mixed with INDO-1–pulsed, purified CD8⁺ T cells derived from TCR-transgenic control or CD2-deficient mice, and centrifuged together. Elevation of [Ca²⁺]_i was assessed by measuring the FL5/FL4 ratio. (A) FL5/FL4 ratio is shown after stimulation with 10⁻¹¹ M p33-pulsed macrophages. (B) Mean FL5/FL4 ratios are shown as a function of the peptide concentration for the various combinations. Baseline FL5/FL4 values were subtracted for the calculation. One representative experiment of two is shown. ▪, CD2⁺ ICAM⁺; •, CD2⁻ICAM⁺; □, CD2⁺ICAM⁻; ○, CD2⁻ICAM⁻.