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. 1999 Dec 20;190(12):1857–1868. doi: 10.1084/jem.190.12.1857

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Primer design and control reaction for the detection of latent messages. (A) The schematic outlines the genomic organization and message structure as determined previously 17. Arrows indicate a given primer identified by its number. SD and SA denote the splice donor and splice acceptor sites, respectively. 71, 72, and 73 refer to the latent orfs of KSHV. (B) The autoradiograph of a Southern hybridization of an RT-PCR reaction using forward primer #7209 and the different primers indicated directly above each lane, which were then hybridized with a latency-specific probe. The reactions used BCBL-1–derived RNA in either the absence (RT−) or presence (RT+) of RT or with no template (water). MW, 100-bp molecular mass markers with the hybridized marker.

Primer design and control reaction for the detection of latent messages. (A) The schematic outlines the genomic organization and message structure as determined previously 17. Arrows indicate a given primer identified by its number. SD and SA denote the splice donor and splice acceptor sites, respectively. 71, 72, and 73 refer to the latent orfs of KSHV. (B) The autoradiograph of a Southern hybridization of an RT-PCR reaction using forward primer #7209 and the different primers indicated directly above each lane, which were then hybridized with a latency-specific probe. The reactions used BCBL-1–derived RNA in either the absence (RT−) or presence (RT+) of RT or with no template (water). MW, 100-bp molecular mass markers with the hybridized marker.