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. 1999 Dec 20;190(12):1857–1868. doi: 10.1084/jem.190.12.1857

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© 1999 The Rockefeller University Press

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Primer design for quantitative RT-PCR. (A) The schematic outlines the genomic organization and message structure for orfs 71, 72, and 73 as determined previously 17. Arrows indicate given primers. (B) The schematic outlines the genomic organization and message structure of orf29 as determined previously 27. Arrows indicate given primers. C and D show detection levels for latent (C, •) and lytic (D, ♦) probes compared with c-myc (□). The number of latent BCBL-1 cells per reaction is shown on the vertical axis, and the corresponding ct value as determined by real-time quantitative RT-PCR is shown on the horizontal axis.

Primer design for quantitative RT-PCR. (A) The schematic outlines the genomic organization and message structure for orfs 71, 72, and 73 as determined previously 17. Arrows indicate given primers. (B) The schematic outlines the genomic organization and message structure of orf29 as determined previously 27. Arrows indicate given primers. C and D show detection levels for latent (C, •) and lytic (D, ♦) probes compared with c-myc (□). The number of latent BCBL-1 cells per reaction is shown on the vertical axis, and the corresponding ct value as determined by real-time quantitative RT-PCR is shown on the horizontal axis.