Table 1.

Primers Used for Detection of Latent and Lytic Transcripts

Latent RT-PCR
#7209	(23mer)	5′-GCATATGCGAAGTAAGAGATTGT
#7307	(21mer)	5′-AGCAGCAGCTTGGTCCGGCTG
#7322	(24mer)	5′-GGTCCTGGGGACTCTCCACAGGAA
#7324	(25mer)	5′-AGCAGCTTGGTCCGGCTGACTTATA
orf29 RT-PCR
#290A	(19mer)	5′-GCAGCTAGCCAACTCCGTG
#290B	(19mer)	5′-GCAGGAAACTCGTGGAGCG
GAPDH control
gapdh-a	(24mer)	5′-CCACCCATGGCAAATTCCATGGCA
gapdh-s	(24mer)	5′-TCTAGACGGCAGGTCAGGTCCACC
Real-time quantitative RT-PCR for spliced lytic orf29
tac29-5F	(16mer)	5′-CCCGGAGGACGGTCCA
tac29-68R	(21mer)	5′-CCCCGAATGCTCTGTTCTTATT
tac29-22T	(23mer)	5′-Fam-CTCGCTGATGTGCGCAACATGCT-Tamra
Real-time quantitative RT-PCR for unspliced latent orf73
lat-273F	(20mer)	5′-ACTGAACACACGGACAACGG
lat-335R	(19mer)	5′-CAGGTTCTCCCATCGACGA
lat-294T	(22mer)	5′-Fam-TAGCGTACTCTCGCGGCCCAGC-Tamra
Real-time quantitative RT-PCR for spliced latent orf72
lat-335R	(19mer)	5′-CAGGTTCTCCCATCGACGA
TAQ-F1	(21mer)	5′-AGGCAGCTGCGCCACGAAGCA
lat-294T	(22mer)	5′-Fam-TAGCGTACTCTCGCGGCCCAGC-Tamra
Ribosomal RNA
riboF	(20mer)	5′-CGGCTACCACATCCAAGGAA
riboR	(18mer)	5′-GCTGGAATTACCGCGGCT
riboP	(22mer)	5′-Joe-TGCTGGCACCAGACTTGCCCTC-Tamra
myc
c-mycF	(20mer)	5′-TCAAGAGGTGCCACGTCTCC
c-mycR	(22mer)	5′-TCTTGGCAGCAGGATAGTCCTT
c-mycP	(23mer)	5′-Fam-CAGCACAACTACGCAGCGCCTCC-Tamra