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. 1999 Dec 20;190(12):1879–1890. doi: 10.1084/jem.190.12.1879

Figure 8.

Figure 8

Caspase and substrate cleavage occur in nonapoptotic cells. (A) Caspase-3 and PARP processing are detected in AV− cells. PBMCs were stimulated (+) or not (−) with anti-CD3 antibody and IL-2 for 4 d, and stained for 10 min with FITC-conjugated AV. Living (AV−) and dying (AV+) activated lymphocytes were purified by flow cytometric cell sorting. Proteins from sorted cells were subjected to Western blot analysis for caspase-3 and PARP. Both antibodies were used on the same membrane. One representative experiment of three is shown. (B) Caspase-6 and -7 and the caspase substrates are processed in viable sorted cells. Unstimulated and unsorted PBMCs (lane 1) or activated and AV− sorted cells (lane 2) were lysed in Laemmli buffer. Proteins from 5 × 105 cells were analyzed by Western blot for PARP, DFF45, Wee1, caspase-6, or caspase-7 processing using the appropriate antisera. Arrows on the right indicate the full-length form of each protein. Results are representative of two independent experiments.