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. 1999 Dec 20;190(12):1825–1836. doi: 10.1084/jem.190.12.1825

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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TS stimulates IL-6 release in T-24 cells via its TR. (A) Dose–response. T-24 cells were stimulated for 24 h with the indicated concentrations of recombinant LTR or CD domains of TS. IL-6 in the conditioned supernatants was assayed by ELISA. Similar results were obtained with PBMCs. (B) Immunodepletion of LTR. An LTR solution was passed through a protein G–Sepharose column adsorbed with either anti-LTR mAb TCN-2 (TCN-2) or control anti–p-azo-phenylarsonate IgG1 (Ctrl/IgG1). The flow-through of both columns was added to T-24 cells for 24 h, and the resulting conditioned media were assayed for IL-6 by ELISA. Eluate for each column was obtained by mechanical stirring of the agarose followed by centrifugation. Eluates were tested for IL-6–secretory activity in the same way as the flow-through.

TS stimulates IL-6 release in T-24 cells via its TR. (A) Dose–response. T-24 cells were stimulated for 24 h with the indicated concentrations of recombinant LTR or CD domains of TS. IL-6 in the conditioned supernatants was assayed by ELISA. Similar results were obtained with PBMCs. (B) Immunodepletion of LTR. An LTR solution was passed through a protein G–Sepharose column adsorbed with either anti-LTR mAb TCN-2 (TCN-2) or control anti–p-azo-phenylarsonate IgG1 (Ctrl/IgG1). The flow-through of both columns was added to T-24 cells for 24 h, and the resulting conditioned media were assayed for IL-6 by ELISA. Eluate for each column was obtained by mechanical stirring of the agarose followed by centrifugation. Eluates were tested for IL-6–secretory activity in the same way as the flow-through.