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. 1999 Dec 20;190(12):1793–1800. doi: 10.1084/jem.190.12.1793

Figure 2.

Figure 2

Isolation of a cDNA coding for the antigen recognized by CTL 361A/21. (A) MHC restriction of CTL 361A/21. CTL activation was followed by measuring the production of TNF after stimulation with autologous tumor cells in the presence of the indicated anti-HLA mAbs. The HLA typing of patient LE9211 is HLA-A3, -B7, -B35, -Cw4, and -Cw7. MZ2-MEL is an allogeneic melanoma line that was used as a negative control. Antibodies W6/32 (anti–HLA class I), 4E (anti–HLA-B/C), ME1 (anti–HLA-B7), and GAPA3 (anti–HLA-A3) were used as described (reference 13). 3,000 CTLs were mixed with 30,000 tumor cells and IL-2 (25 U/ml), and TNF production was measured 18 h later as described elsewhere (reference 11). (B) cDNA 4.1 cloned into plasmid pcDNAI/Amp was transfected into COS cells together with the HLA-B*0702 cDNA cloned into plasmid pcDSRalpha. CTL 361A/21 (3,000 cells) was added after 24 h, and TNF production was measured 18 h later.