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Recognition of normal kidney cell lines by CTL 361A/21. LB2043-PTEC and LB2046-PTEC are two short-term cell lines established from normal kidney samples and corresponding to epithelial cells of the proximal tubule. They were transiently transfected with the indicated HLA cDNAs before the addition of 10,000 CTL 361A/21 in the presence of 25 U/ml IL-2. The presence of IFN-γ in the supernatant was measured by ELISA after an overnight incubation.
