FIG. 7.
Inhibition of polyC9 deposition on ER by paramyosin. Fresh ER were treated with 100 μl of NHS supplemented with 6 μg of C9 preincubated for 30 min at 37°C with (lane a) or without (lane b) 40 μg of recombinant paramyosin. The lysed ER were sedimented, and the pellet was dissolved in reducing sample buffer and analyzed on a SDS-PAGE gradient gel (3 to 10% acrylamide) stained with Coomassie blue. Lane m, molecular mass markers (200, 120, 97, and 67 kDa). The arrow indicates a band of polyC9. Other bands represent proteins originated from the red blood cells and serum proteins adhering to blood cells.