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. 1999 Dec 6;190(11):1617–1626. doi: 10.1084/jem.190.11.1617

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© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Representative flow cytometric profiles of cells derived from single FT progenitors. (A) Procedure of the clonal assay culture is shown. Single CD44⁺CD25⁻ FT cells (14 dpc) were picked up under microscopical visualization, then seeded into the wells where a dGuo-treated FT lobe had been placed. The culture medium used was supplemented with a cytokine cocktail (see legend to Fig. 1). Cells were cultured under HOS conditions for 10 d. (B) Examples of the flow cytometric profiles of cells generated from p-NK, p-T, and p-T/NK are shown. Cell recoveries in these clones were 2.4 × 10⁴, 8.0 × 10⁴, and 2.0 × 10⁵, respectively.