Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 1999 Dec 6;190(11):1697–1710. doi: 10.1084/jem.190.11.1697

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 1999 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Increased Ig, RF, and CIC levels in BAFF-Tg mice. (A) Reduced SDS-PAGE of sera from five control littermates and nine BAFF-Tg mice showing that BAFF increases IgG levels. For comparison, mouse IgG1 (MOPC-21) was included as a standard: loading per lane was 5 μg of MOPC-21 and 0.5 μl of the serum. The sharp band slightly below the Ig light chain is not an immunoglobulin and the IgM heavy chain comigrates with transferrin. ELISA-based analysis of total mouse Ig (B), RF (C), and CIC (D) in the sera of 19 control littermates (white bars) and 21 BAFF-Tg mice (black bars). The titer (log base 2) for RF is defined as the dilution of the sera giving an OD three times higher than that of background. The quantity of CIC is defined as the quantity of peroxidase-mouse antiperoxidase required to generate an OD equivalent to that obtained with the tested serum. The difference between control animals and BAFF-Tg mice was statistically significant (P < 0.001 in B and C, P < 0.003 in D).

Increased Ig, RF, and CIC levels in BAFF-Tg mice. (A) Reduced SDS-PAGE of sera from five control littermates and nine BAFF-Tg mice showing that BAFF increases IgG levels. For comparison, mouse IgG1 (MOPC-21) was included as a standard: loading per lane was 5 μg of MOPC-21 and 0.5 μl of the serum. The sharp band slightly below the Ig light chain is not an immunoglobulin and the IgM heavy chain comigrates with transferrin. ELISA-based analysis of total mouse Ig (B), RF (C), and CIC (D) in the sera of 19 control littermates (white bars) and 21 BAFF-Tg mice (black bars). The titer (log base 2) for RF is defined as the dilution of the sera giving an OD three times higher than that of background. The quantity of CIC is defined as the quantity of peroxidase-mouse antiperoxidase required to generate an OD equivalent to that obtained with the tested serum. The difference between control animals and BAFF-Tg mice was statistically significant (P < 0.001 in B and C, P < 0.003 in D).