Skip to main content
. 1999 Dec 6;190(11):1689–1696. doi: 10.1084/jem.190.11.1689

Figure 2.

Figure 2

Radioimmunoassay for the detection of anti–mouse RBC activities by the 4C8 (IgM and IgG2a) and 34-3C (IgG2a) anti–mouse RBC mAbs. RBCs freshly prepared from BALB/c mice were first incubated with different concentrations of various mAbs, and then incubated with radiolabeled goat anti–mouse Ig. The results are expressed as the radioactivity bound to mouse RBCs in the presence of 4C8 IgM (•), 4C8 IgG2a (□), and 34-3C IgG2a (○). As controls, the results obtained with isotype-matched control mAbs are shown: IgM anti-IgG2a (▪) and IgG2a anti-TNP (▵).