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. 2000 Jan 17;191(2):365–374. doi: 10.1084/jem.191.2.365

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© 2000 The Rockefeller University Press

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Creation of OX40L-deficient mice. (A) Structure of the wild-type and mutant OX40L alleles. The targeting construct was designed to replace the first exon (black box) with a PGKneo gene cassette. The location of the probe for hybridization, the 0.8-kb PvuII-StuI fragment, is shown (hatched box). (B) Southern blot analysis of offspring of the germline chimera. Tail DNA harvested from wild-type, OX40L^+/−, and OX40L^−/− mice was digested with HindIII (the location of which is depicted as H in A), electrophoresed, and probed with the radiolabeled probe. The mutant and wild-type alleles gave 10.0- and 3.0-kb hybridizing bands, respectively. (C) FACS^® analysis of surface expression of OX40L. Splenic B cells from wild-type and OX40L^−/− mice were stimulated with anti-CD40 plus anti-IgM, and then stained with anti–mouse OX40L mAb, MGP34.