FIG. 3.

Induction of tracheal antimicrobial peptide mRNA in bovine tracheal epithelial cells stimulated for 18 h with lipopolysaccharide (LPS) or with various concentrations of rhLf-K or rhLf-R as determined by RT-PCR. PCR products amplified with tracheal antimicrobial peptide (TAP)-specific primers were visualized by autoradiography. PCR products amplified with glyceraldehyde-3-phosphate dehydrogenase (GAPDH)-specific primers were visualized by staining with ethidium bromide.