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. 2001 Feb 19;152(4):741–752. doi: 10.1083/jcb.152.4.741

Figure 1.

Figure 1

Figure 1

Figure 1

Activation of ERK1 and ERK2 in uPA–PAI-1 complex-treated MCF-7 cells. MCF-7 cells were serum starved for 12 h and then treated with 5 nM DIP–uPA (A), 5 nM uPA–PAI-1 complex (B), or vehicle (Control; A and B) for the indicated times. (C) Cultures were incubated with uPA- or uPAR-specific antibodies (+) or vehicle (−) for 15 min at 37°C and then exposed to uPA–PAI-1 complex (5 nM) or vehicle for 5 min. Phosphorylated ERK1 and ERK2 were detected by immunoblot analysis. The nitrocellulose membranes were then stripped and reprobed with a separate antibody that detects total ERK.