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. 2001 Feb 19;152(4):705–716. doi: 10.1083/jcb.152.4.705

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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SMA and insoluble LEN do not progress in folding. Transfected cells were treated with 10 μg/ml ALLN overnight. Soluble (S) and pelleted, insoluble (P) fractions were separated by SDS-PAGE under reducing (R) and nonreducing (NR) conditions and analyzed by Western blotting. Twofold more insoluble than soluble material was loaded. F₀, completely reduced LC; F_ox, LC containing two disulfide bonds. Only soluble LEN folds into the F_ox state. All of SMA, insoluble LEN, and a small percentage of soluble LEN is found in the F₀ state.