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. 2001 Feb 19;152(4):693–703. doi: 10.1083/jcb.152.4.693

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Expression of MMPs and TIMPs during adipocyte differentiation. (a) RNA was extracted from cultures of 3T3-L1 cells at various stages of differentiation. pre, subconfluent fibroblastic precursor; com, confluent, committed stage at day 0 of differentiation; 2–8 dD, 2–8 d differentiation after administration of DM. RNA was separated by agarose gel electrophoresis and transferred to membranes for analysis of expression of specific MMP and inhibitor mRNAs. Autoradiograms were scanned and the relative expression of specific mRNAs is a ratio of EtBr-stained 18S rRNA was quantified. (b) Enzymes secreted by adipocytes into supernatant media were analyzed by gelatin zymography. Gelatinase A, identified by comparison with mouse enzyme standards, was progressively upregulated and activated (*) as 3T3-L1 cells differentiated.