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. 2001 Feb 19;152(4):809–824. doi: 10.1083/jcb.152.4.809

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Mature melanosomes in MNT-1 cells are not accessed by endocytic tracers. (A) BSAG (5-nm) was internalized for 30 min. BSAG is present in coated endosomes and multivesicular bodies (arrowheads) situated near mature melanosomes labeled for TRP1 (PAG 10) but does not label the melanosomes. (B) BSAG (5-nm) internalized for 2 h accumulates in electron-dense lysosomes but not in TRP1-positive (PAG 10) melanosomes (IV). Bars, 100 nm.